Cell Respiration with Germinating Pea Plants
Purpose:
The purpose of this lab is to calculate the rate of cell respiration in germinating and non-germinating peas.
Hypothesis:
If we measure the rate of respiration between germinating and non-germinating pea plants, then the germinating pea plant will have a faster rate of respiration than the non-germinating peas and beads.
Materials:
Water Bath
25 dormant peas
25 germinating peas
3 respirometers
25 glass beads
Thermometer
15 % KOH
3 pipettes
3 rubber
stoppers
Tape
Thermometer
6 Cotton Balls
3 Glass Vials
Procedure:
1. Fill the water bath with water to allow the water inside the bath to become a stable temperature.
2. Next, in order to set up the respirometer, obtain a glass vial.
3. Place one cotton ball at the bottom of each glass vial.
4. Using an eyedropper, drop about 2-3 drops of KOH on the first cotton ball. After doing so, place a second
cotton ball onto the absorbent cotton.
5. Repeat for the three respirometers.
6. Label your respirometers. In the first respirometer, place germinating peas. In the next one, place the dry peas into it. Lastly, place beads in the third respirometer. The beads will serve as the control since they obviously do not respirate.
7. Place a rubber stopper on each glass vial. Then, place a pipette wrapped with tape in each of the stoppers to ensure that no air can enter the vial.
8. Place tape on the water bath to make a netlike modality to hold the vials out of the water.
9. Place the respirometers tilted at an angle out of the water on a sling and allow them to sit for a five minute period where they will equalize.
10. Place respirometers into the water bath and allow equalizing for another 5 minutes. Begin recording position of the water in the pipette in divided intervals.
Purpose:
The purpose of this lab is to calculate the rate of cell respiration in germinating and non-germinating peas.
Hypothesis:
If we measure the rate of respiration between germinating and non-germinating pea plants, then the germinating pea plant will have a faster rate of respiration than the non-germinating peas and beads.
Materials:
Water Bath
25 dormant peas
25 germinating peas
3 respirometers
25 glass beads
Thermometer
15 % KOH
3 pipettes
3 rubber
stoppers
Tape
Thermometer
6 Cotton Balls
3 Glass Vials
Procedure:
1. Fill the water bath with water to allow the water inside the bath to become a stable temperature.
2. Next, in order to set up the respirometer, obtain a glass vial.
3. Place one cotton ball at the bottom of each glass vial.
4. Using an eyedropper, drop about 2-3 drops of KOH on the first cotton ball. After doing so, place a second
cotton ball onto the absorbent cotton.
5. Repeat for the three respirometers.
6. Label your respirometers. In the first respirometer, place germinating peas. In the next one, place the dry peas into it. Lastly, place beads in the third respirometer. The beads will serve as the control since they obviously do not respirate.
7. Place a rubber stopper on each glass vial. Then, place a pipette wrapped with tape in each of the stoppers to ensure that no air can enter the vial.
8. Place tape on the water bath to make a netlike modality to hold the vials out of the water.
9. Place the respirometers tilted at an angle out of the water on a sling and allow them to sit for a five minute period where they will equalize.
10. Place respirometers into the water bath and allow equalizing for another 5 minutes. Begin recording position of the water in the pipette in divided intervals.
Results:
Theoretical data of germinating pea lab at different temperatures.
How it relates:
My hypothesis was supported by the data I collected in this experiment:), that the germinating peas would require the most oxygen because they are trying to grow. My data also supported that the glass beads, a non-living thing would not require any oxygen.
This lab demonstrates how organisms use oxygen at different rates and that non-living organisms do not use oxygen.
Problem Faced:
-the vials once place are supposed to be left undisturbed, but our vials had to be adjusted because the tape was falling into the water, so we had to disturb the vials and adjust the tape. This may have caused more oxygen to seep out of the vials, which may have skewed our results.
Error Analysis:
Several factors could have caused inaccurate results in this experiment.
-not maintaining a constant temperature
-moving the vials in the water after the experiment began
-Putting our hands in the water bath while the vials were in the water
-Allowing the peas to come into contact with the KOH
-not having the same amount of cotton in each vial could have caused an error in the results.
The experiment had it's errors, but the data still allowed us to
understand the purpose.
Conclusion:
My hypothesis was supported by the data I collected in this lab!:)
In this experiment the vial with just germinating peas had the greatest consumption of oxygen, because germinating peas carried out a faster rate of cellular respiration.
The beads carried out no cellular respiration because they are not living.
The dry peas carry out a slower process of cellular respiration.